Fig. 5

Effects of aclidinium bromide (Acl) and fluticasone propionate (Flut) on lipopolysaccharide (LPS)-induced corticosteroid modulators. Human peripheral blood neutrophils isolated from COPD patients were plated on 24-well plates, incubated with Acl, Flut or a combination thereof for 1 h, and stimulated with LPS for 6 h. Molecular corticosteroid modulators were quantified by RT-PCR using the 2^−ΔCt method, with expression of the housekeeping gene GAPDH serving as an internal control. The results are expressed as the mean ± SD (n = 4 each for cells from healthy controls and COPD patients in independent experiments with triplicate samples). A two-way ANOVA was followed by a post hoc Bonferroni test. *p < 0.05 vs. control unstimulated cells; #p < 0.05 vs. Flut monotherapy