Fig. 2From: Administration of JTE013 abrogates experimental asthma by regulating proinflammatory cytokine production from bronchial epithelial cellsS1P stimulation of airway ECs induces CCL3 and TIMP2 gene expression, and CCL3 and TIMP2 are S1P-dependent in vitro. BEAS-2B cells were cultured with or without S1P (100 nM). The mRNA expression of 29 cytokines was analyzed by quantitative real-time RT-PCR. Data represent the ratio between the relative mRNA level of S1P-treated cells and that of S1P-untreated cells (a). BEAS-2B cells were treated with S1P (100 nM or 1 μM) (b), S1P (1 μM) and JTE013 (10 μM), or S1P (1 μM) and VPC23019 (10 μM) (c) for 3 h, and CCL3 and TIMP2 gene expression was analyzed by quantitative real-time RT-PCRBack to article page