The impact of low-frequency, low-force cyclic stretching of human bronchi on airway responsiveness

Respiratory Research

Table 2 Effects of pretreatments on the post-stretch response in bronchial tone

		Cyclic stretch effect on post-stretch response, difference between control and stretch group		Effect of pretreatment	Effect size pretreatment
	n	in absence of pretreatment (g)	in presence of pretreatment (g)	(g)	\|d\| [95% CI]
Epithelium removal	13	0.24 ± 0.03^a	0.15 ± 0.03^a	−0.09 ± 0.03^d	−3.00 [−4.01– − 1.80]
Indomethacin (1 μ M)	8	0.36 ± 0.07^b	0.29 ± 0.07^b	−0.07 ± 0.11	−0.37 [−1.34–0.64]
MK476 (1 μM)	7	0.35 ± 0.08^b	0.23 ± 0.06^c	−0.12 ± 0.10	−0.64 [−1.67–0.47]
L-NAME (1 mM)	14	0.29 ± 0.03^a	0.21 ± 0.03^a	−0.08 ± 0.02^d	−0.73 [−1.47–0.06]
Nicardipine (10 μM)	10	0.17 ± 0.03^a	0.12 ± 0.08^a	−0.05 ± 0.07	−0.27 [−1.13–0.63]
Tetrodotoxin (1 μM)	7	0.37 ± 0.08^b	0.29 ± 0.06^b	−0.07 ± 0.10	−0.43 [−1.46–0.66]
Gadolinium (0.1 mM)	8	0.36 ± 0.07^b	0.26 ± 0.03^a	−0.10 ± 0.08	−0.69 [−1.65 − 0.36]
Tertiapine (10 μM)	9	0.23 ± 0.06^b	0.27 ± 0.04^a	0.04 ± 0.08	0.26 [−0.68–1.18]
Capsazepine (1 μM)	8	0.29 ± 0.06^b	0.37 ± 0.05^a	0.08 ± 0.06	0.74 [−0.31–1.71]
Calphostine C (0.1 μM)	9	0.21 ± 0.03^a	0.26 ± 0.02^a	0.05 ± 0.05	0.65 [−0.32–1.57]
SB203580 (0.1 μM)	10	0.29 ± 0.05^a	0.15 ± 0.19	−0.14 ± 0.22	−0.32 [−1.19–0.58]
Y27632 (1 μM)	11	0.28 ± 0.05^a	0.33 ± 0.07^b	0.05 ± 0.08	0.25 [−0.60–1.08]

The post-stretch response in bronchial tone corresponded to the spontaneous post-stretching increase within 10 min following the cyclic stretch period (difference between the points 5 and 4, Fig. 1). Values are means ± standard error of the mean and standardized effect size |d| and its 95% confidence interval [CI] for the difference between means. The observed effect of pretreatment is small (IdI ≥ 0.20), medium (IdI ≥ 0.50), or large (IdI ≥ 0.80) according to the Cohen’s conventions [23]. The 95% CI for |d| consists of the uncertainty around the real effect of pretreatment. ^a P < 0.001, ^b P < 0.01, ^c P < 0.05 stretched vs. paired non-stretched control bronchi. ^d P < 0.05 pretreatment vs. no pretreatment

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