Fig. 4
From: Increased AGE-RAGE ratio in idiopathic pulmonary fibrosis
Immunostaining of RAGE in control (A) and IPF (B) lung sections. a1 Parenchyma show a strong brown staining, focused in the alveolar epithelium. a2 Pneumocytes show brown staining over its cell membrane (arrow), although there is a discontinuous pattern. a3 Ciliated epithelial cells of bronchus show a weak staining pattern. a4 Endothelium and smooth muscle of veins do not show brown staining. b1 Areas of matrix deposition do not show immunoreactivity. b2 Preserved parenchyma show a weak staining (arrow). b3 Fibroblast foci (star) and AECs (arrow) that surround these fibroblast foci show almost undetectable immunostaining. b4 No signal was considered in blood vessels and preserved bronchus. Micrographs were recorded at 100X (b1 and 4), 200X (a1, b2 and 3) and 400X magnification (a2, 3 and 4)