Fig. 2

Pentosidine and CML expression by Western blot. a1 Anti-pentosidine antibody used for Western blot assessment showed a dissimilar pattern of bands at 45 and 30 kDa. Two different pentosidine-modified proteins were recognized. a2 Densitometry analyses showed a major presence of pentosidine in the IPF group compared with the control, for both bands. b1 Anti-CML antibody showed several patterns of bands of CML-modified proteins: a high band in 109 kDa, and two bands in 69 and 58 kDa. CML at 109 and 58 kDa are stronger in IPF than control lungs. b2 Densitometry analyses showed a major presence of CML-contained proteins in the IPF group in comparison to the control. Data were represented as percentage in relation to loading control α-tubulin (α-tub) and β-actin (β-act), respectively. Statistical analysis was performed using Mann–Whitney U-test and Student’s t-test, respectively (**p < 0.01)