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Fig. 8 | Respiratory Research

Fig. 8

From: Proteases and oxidant stress control organic dust induction of inflammatory gene expression in lung epithelial cells

Fig. 8

Effects of α1-antitrypsin and CDDOIm on the induction of IL-8 promoter activity. a A549 cells were transfected with an IL-8 promoter plasmid containing −144/+44 bp of human IL-8 promoter sequence linked to luciferase reporter gene. Transfected cells were treated with medium (C), 0.25 % dust extract (DE), 10 μg/ml α1-antitrypsin alone (α1-AT), or 0.25 % dust extract in the presence of 10 μg/ml α1-antitrypsin for 6 h. Luciferase activities in cell lysates were measured and normalized to total cell protein. Luciferase activity in untreated cells (C) was arbitrarily set as 100. Data shown are means ± SE (n = 4). *P < 0.05. b and c After transfection with an IL-8 promoter plasmid (−144/+44 bp), A549 (b) and Beas2B (c) cells were incubated first with medium (C) or 1-(2-Cyano-3,12,28-trioxooleana-1,9(11)-dien-28-yl)-1H-imidazole (CDDOIm) (1 μM for A549 and 0.5 μM for Beas2B) for 3 h and then treated with or without dust extract (0.25 %) (DE) for 6 h. Luciferase activities were normalized to total cell protein. Data shown are means ± SE (n = −3). *P < 0.05 and **P < 0.01

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