Fig. 6From: Proteases and oxidant stress control organic dust induction of inflammatory gene expression in lung epithelial cellsEffects of antioxidants on the induction of inflammatory gene mRNA levels. Beas2B (a–c) and SAE (d–f) cells were first incubated with medium or medium containing 30 mM DMTU for 1 h or 15 mM n-acetylcysteine (NAC) adjusted to pH 7 or 0.5 μM 1-(2-Cyano-3,12,28-trioxooleana-1,9(11)-dien-28-yl)-1H-imidazole (CDDOIm) for 3 h and then treated with medium or 0.25 % dust extract (DE) for 3 h. Levels of mRNAs were determined by qRT-PCR. Levels of mRNAs in dust extract treated cells were arbitrarily considered as 100, and relative levels in cells treated with a combination of antioxidant and dust extract are shown. Data shown are means ± SE (n = 3–4) except in the case of TLR4 for Beas2B cells treated with a combination of CDDOIm and DE, in which case data is shown as mean ± SD (n = 2). *P < 0.05; **P < 0.01; ***P < 0.001, #P < 0.0001 compared with cells treated with dust extract aloneBack to article page