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Table 2 Primer sequences used for real-time quantitative PCR of mRNA and miRNA

From: Sirt1 expression is associated with CD31 expression in blood cells from patients with chronic obstructive pulmonary disease

Target gene Upper-primer Lower-primer
CD31 5′-AACCCACTCCCCGACCTAGA-3′ 5′-CCAGACACCATTCCAAAACC-3′
CD34 5′-TAAGAAGGACAGGGGAGAGG-3′ 5′-GCCAAGACCAGCAGTAGACA-3′
Sirt1 5′-GGGGTGTCTGTTTCATGTGG-3′ 5′-ACATCGCTTGAGGATCTGGA-3′
IL-6 5′-GGTACATCCTCGACGGCATC-3′ 5′-TGCCTCTTTGCTGCTTTCAC-3′
p21 5′-GGAAGACCATGTGGACCTGT-3′ 5′-GGCGTTTGGAGTGGTAGAAA-3′
18 s rRNA 5′-GACTCAACACGGGAAACCTC-3′ 5′- CGCTCCACCAACTAAGAACG-3′
miR-34a 5′-GGCAGTGTCTTAGCTGGTTGT-3′  
miR-126-3p 5′-TCGTACCGTGAGTAATAATGC-3′  
miR-103 5′-CAGCATTGTACAGGGCTATGA-3′