Fig. 8

Effect of CSE on gene and protein expression in cultured human pulmonary microvascular endothelial cells (HPMVECs). Cultured HPMVECs were exposed to 0.3 % CSE for 24 h, and Sirt1, CD31 mRNA, miR-34a and miR-126-3p expressions were measured by RT-PCR analysis (a). Sirt1 and p53 protein expression was measured by western blot analysis (b). CSE treatment significantly suppressed Sirt1 mRNA and protein expression and increased miR-34a and p53 protein expression. Data are expressed as mean ± SD (n = 6 for RT-PCR analysis, n = 4 for western blot analysis). *P < 0.05 versus control