Fig. 5

Expression of apical TLR4 and EGFR, and nuclear phosphorylated-ERK1/2 is augmented in LPS-pretreated athymic mice. a Quantification of the number of AECs with apical TLR4 and EGFR and representative micrographs of axial airways from S/0 and L/0 Foxn1^WT and Foxn1^nu mice. The panels are stained for DAPI (blue) nuclei, TLR4 (red), EGFR (green) and a merged image. b Quantification of the number of AECs with nuclear pERK in Foxn1^WT and Foxn1^nu mice. Representative micrographs of axial airways stained for pERK (green) and DAPI-stained (blue) nuclei from S/0 and L/0 Foxn1^WT and Foxn1^nu mice are shown. Error bar indicates mean ± SEM (n = 6 per group). * P < 0.05, ** P < 0.01 compared to Foxn1^WT S/0 unless otherwise indicated