Fig. 1From: A HuR/TGF-β1 feedback circuit regulates airway remodeling in airway smooth muscle cellsExperimental protocols for chronic OVA-induced asthma in mice. BALB/c mice received three intraperitoneal sensitizations with ovalbmin grade V mixed with the adjuvant aluminium hydroxide for emulsified on days 0, 7 and 14, followed by seven consecutive challenge each day with 5 % ovalbumin grade III aerosols for 30 min. The negative control mice were received the same volume of phosphate-buffered saline (PBS) by intraperitoneal injection for three times, then undergo airway challenge. No exogenous adjuvant was given at any time. The mice were sacrificed with an over dose anaesthetized, BALF was performed, and the lungs were dissected for histological analysisBack to article page