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Fig. 12 | Respiratory Research

Fig. 12

From: Vitamin D and allergic airway disease shape the murine lung microbiome in a sex-specific manner

Fig. 12

Allergic airway disease had a substantially greater effect on bronchoalveolar lavage neutrophil numbers than vitamin D deficiency. Neutrophil and macrophage numbers in the bronchoalveolar lavage (BALF) and blood of naïve mice fed a vitamin D (VitD)-replete (+) or -deficient (−) or initially vitamin D-deficient then replete diet (−/+) diet were compared with those of vitamin D-replete mice with OVA-induced allergic airway disease, 24 h after respiratory challenge with OVA. For OVA-sensitisation, mice were injected with OVA and Alum at 12 weeks of age, boosted with OVA and Alum at 14 weeks of age, and then administered a respiratory challenge with OVA at 15 weeks of age. BALF or blood was obtained 24 h later. In (a-d) results from males, and (e-h) results from female mice. In (a) and (e) neutrophils, and (b) and (f) macrophages in BALF are shown (n ≥ 14 mice/treatment). In (c) and (g) neutrophils, and (d) and (h) monocytes per ml of blood are depicted (n = 5–10 mice/treatment). Data is shown as mean + SEM (*p < 0.05)

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