Fig. 10
From: Vitamin D and allergic airway disease shape the murine lung microbiome in a sex-specific manner
Ovalbumin-induced allergic airway disease substantially modified the lung microbiome. The lung microbiomes of naïve mice (naïve, n = 56) were compared with those with ovalbumin (OVA)-induced allergic airway disease (OVA, n = 68), 24 h after respiratory challenge with OVA, with results combined for all naïve or all OVA mice. To induce allergic airway disease, mice were injected with OVA and Alum at 12 weeks of age, boosted with OVA and Alum at 14 weeks of age, and then administered a respiratory challenge with OVA at 15 weeks of age. Lungs were obtained 24 h later. In (a), a non-metric multidimensional scaling (NMDS) plot depicts the dissimilarity of the detected lung of operational taxonomic units (OTUs) for all mice, and in (b) for mice only fed the vitamin D-supplemented diet (+). (M = male, F = female; n = 9–10/treatment)