Serum extracellular vesicular miR-21-5p is a predictor of the prognosis in idiopathic pulmonary fibrosis

Respiratory Research

Table 2 Serum EV microRNAs that showed a significant* increase in the bleomycin-induced lung fibrosis model compared with non-treated controls

Day 7	Fold increase	Day 14	Fold increase	Day 28	Fold increase
miR-17-3p	12.33	miR-21-5p	5.77	miR-21-5p	7.97
miR-21-5p	9.29	miR-155-5p	3.26	miR-122-5p	7.24
miR-130b-3p	7.50	miR-106a-5p	2.27	miR-155-5p	6.37
miR-134-5p	4.81			miR-574-3p	3.53
miR-155-5p	4.28			miR-125b-5p	3.40
miR-19a-3p	4.10
miR-122-5p	4.07
miR-19b-3p	4.07
miR-221-3p	3.64
miR-106b-5p	3.51
miR-22-3p	3.20
miR-423-5p	3.09

The expression levels of microRNAs were normalized to the amount of EVs in the mouse serum samples. The Ct values of these microRNAs were less than 30 indicating that the relative expression levels of these microRNAs were sufficiently high to evaluate for fold-changes without excessive variation. Both miR-21-5p and miR-155-5p (indicated by boldface) were significantly increased at every measured time point during the 28 days after bleomycin administration
*q value < 0.05 compared with non-treated control. The q value (false discovery rate) was calculated by the Benjamini-Hochberg procedure

ISSN: 1465-993X