Fig. 5

Loss of RGS2 arguments the development of bleomycin-induced pulmonary fibrosis in mice. a Outline of experimental design. On Day 1, bleomycin was administrated intratracheally into RGS2+/+ and RGS2−/− mice. On Day 22, dynamic lung compliance of mice was measured. b Loss of RGS2 augments the death rate in bleomycin (2 U/kg body weight)-induced mouse pulmonary fibrosis model. Kaplan-Meier survival curves were created for bleomycin-exposed RGS2+/+ and RGS2−/− mice (n = 12 per group). c Effects of bleomycin (1 U/kg) on dynamic lung compliance (C_dyn) of RGS2+/+ and RGS2−/− mice. d The lungs were stained with trichrome to visualize collagen deposition (blue). Representative sections from each group are shown (scale bar = 200 μm). e Sections were scored by the modified Ashcroft method for overall fibrosis. Results are shown as means ± SEM (n = 5) and were analyzed using a two-sided t-test. f Total lung collagen content was measured by the Sirius red collagen assay. Results are expressed as μg of collagen per 100 mg of lung tissue. Data are means ± SEM (n = 5 mice). ** p < 0.01