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Fig. 2 | Respiratory Research

Fig. 2

From: Upregulation of RGS2: a new mechanism for pirfenidone amelioration of pulmonary fibrosis

Fig. 2

Increase of RGS2 attenuates thrombin-induced increase of [Ca2+]i in HFL1 cells. HFL1 cells were infected with Ad-mCherry or Ad-RGS2/mCherry for 24 h. a RGS2 and mCherry expression was assessed by western blots (left panel) and fluorescence microscopy (right panel), respectively. Infection efficiency was approximately 90 % based on the percentage of cells expressing mCherry (scale bar = 50 μm). b Thrombin induced a dose-dependent increase of [Ca2+]i in un-infected HFL1 cells. c Thrombin-induced [Ca2+]i increase in HFL1 cells infected with Ad-mCherry or Ad-RGS2/mCherry. Cells pre-loaded with Fluo-8 were stimulated with various concentrations of thrombin (0.1–2 U/ml) (b) or 1 U/ml of thrombin (c). The increase of Fluo-8 fluorescence from four experiments is plotted as means ± SEM. ** p < 0.01 as compared to control untreated cells

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