Fig. 1

Identification of RGS2 as an early response gene to PFD treatment in human lung fibroblasts. Genechip microarray revealed PFD–induced upregulated genes, with the top ten shown (a) and selective upregulation of RGS2 in the RGS gene family in human fetal lung fibroblast (HFL1) cells (b). Data shown are the means of two Genechip microarray assays using different pooled total RNA. The results are expressed as the fold change calculated as the ratio of the two groups. HFL1, three control primary human lung fibroblast (CPHLF1-3) (c and d), and two diseased primary human lung fibroblast (DPHLF1-2) (e) cell lines were treated with saline (control) or 10 mM PFD for 2 h and then processed for real-time PCR (c, e) and western blot analyses (d, e). The results of real-time PCR were normalized as RGS2/β-actin × 1000 and plotted as means ± SEM (n = 4). A blot representative of three experiments is shown (d and e, insets). Mean RGS2 band density was normalized to β-actin ± SEM. HFL1 cells were treated with various concentrations of PFD (0–10 mM) for 2 h (f) or with 10 mM PFD over a time course of 18 h (g). Cells were harvested for real-time PCR analysis of RGS2 and β-actin mRNA levels. The results are shown as means ± SEM (n = 4). * p < 0.05, ** p < 0.01 vs. control