Fig. 2
From: Induction of pulmonary antibodies against oxidized lipids in mice exposed to cigarette smoke
Impact of anti-OxPL antibody delivery on CS-induced pulmonary inflammation. a Female 6–8 week old C57BL/6 mice were exposed to room air (RA) or cigarette smoke (CS) for 1 h, twice a day for 4 days. They received phosphate-buffered solution (35 μl; PBS), a mouse IgM isotype control (20 μg in 35 μl; ISO), or a mouse monoclonal antibody against oxidized phosphadytilcholine (20 μg in 35 μl; clone E06) intranasally every day before the first cigarette smoke exposure of the day. b Total cells, mononuclear cells and neutrophils were counted in the bronchoalveolar lavage (BAL). c Monocyte chemotactic protein 1 (MCP-1), interleukin-1 alpha (IL-1α), and granulocyte macrophage colony-stimulating factor (GM-CSF) levels were measure in the BAL fluid (BALF) by ELISA. Data represent mean ± SEM; n = 4–5/group; representative of two independent experiments; bars with different superscripts are significantly different from each other