Fig. 4
From: Vimentin dephosphorylation at ser-56 is regulated by type 1 protein phosphatase in smooth muscle
Dissociation of p130CAS from vimentin is regulated by PP1. a Equal amount of insoluble vimentin from untreated and okadaic acid (OA, 10−6 M)-treated tracheal rings were separated by SDS-PAGE, and transferred to nitrocellulose membranes. The membranes were probed with use of p130CAS antibody and vimentin antibody. The ratios of p130CAS to vimentin in the OA-treated tissues are normalized to those in untreated tissues. *Significantly lower ratio of p130CAS/vimentin in treated rings as compared to untreated tissues (p < 0.05). Data are mean ± SE (n = 5). b Tracheal tissues expressing control (Ctrl) or PP1 shRNA were stimulated with 10−4 M ACh for 5 min. The ratios of p130CAS/vimentin in tissues treated with PP1 shRNA are normalized to the ratios in tissues treated with control shRNA. Data are mean ± SE (n = 4). *,p < 0.05