Transduction efficiency after intratracheal injection of CF6 shRNA in vivo. Representative image of GFP expression (a) in lung tissues of rats at 2w after CF6 shRNA or GFP vector injection in sham group, control vector group and CF6 shRNA group (n = 4 for each group). Cell nuclei were labeled with DAPI DNA stain (blue). Bar = 50 μm. b, c CF6 (18 KDa) protein levels normalized by GAPDH (36 KDa), as analyzed by Western blot and relative CF6 mRNA levels, as detected by quantitative RT-PCR in the sham, vehicle, −2w-MCT, −2w-shRNA, 2w-MCT and 2w-shRNA groups. **p < 0.01.