Fig. 4

In vitro exposure to cigarette smoke extract (CSE) of monocyte-derived dendritic cells (MDDC) altered their ability to prime T-cells. MDDC were exposed to CSE and then activated or not by S. pneumoniae (Sp). After 24 h incubation, MDDC were then cocultured 5 days with autologous T-cells. Levels of a IFN-γ, b IL-17, c IL-22 and d IL-10 were quantified by ELISA in coculture supernatants. In some experiments, either recombinant human IL-12 or IL-23 were added to the cocultures with CSE- and Sp-exposed MDDC in order to quantify their ability to restore e IFN-γ or f IL-17 secretion, respectively. Data are reported as mean ± S.E.M. of 14 and 3 experiments for a-d and e-f, respectively. *P < 0.05, **P < 0.01, ***P < 0.001