Differential effect of mild and severe pulmonary embolism on the rat lung transcriptome

Respiratory Research

Table 4 Steroid/Sterol Biosynthesis Genes

Symbol	Fold	Dir.	Gene Name
CH25H	6.84	UP	Cholesterol 25-hydroxylase
DHCR24	2.33	UP	24-dehydrocholesterol reductase
DHCR7	2.15	UP	7-dehydrocholesterol reductase
FDFT1	1.56^a	UP	Farnesyl diphosphate farnesyl transferase 1
FDPS	1.89	UP	Farnesyl diphosphate synthase (farnesyl pyrophosphate synthetase, dimethylallyltranstransferase, geranyltranstransferase)
HMGCR	2.11^a	UP	3-hydroxy-3-methylglutaryl-Coenzyme A reductase
HMGCS1	3.74	UP	3-hydroxy-3-methylglutaryl-Coenzyme A synthase 1 (soluble)
HSD17B12	3.03	UP	Hydroxysteroid (17-beta) dehydrogenase 12
HSD17B7	4.33	UP	Hydroxysteroid (17-beta) dehydrogenase 7
IDI1	8.61	UP	Isopentenyl-diphosphate delta isomerase 1
MVD	3.34	UP	Mevalonate (diphospho) decarboxylase
NSDHL	1.84	UP	NAD(P) dependent steroid dehydrogenase-like
SC4MOL	1.9	UP	Sterol-C4-methyl oxidase-like
SC5DL	2.33^a	UP	Sterol-C5-desaturase (ERG3 delta-5-desaturase homolog, S. cerevisiae)
SOAT1	2.59^a	UP	Sterol O-acyltransferase 1
SQLE	3.26	UP	Squalene epoxidase

Steroid and sterol biosynthesis genes altered in the 18-h Severe PE treatment group. The three steroid/sterol annotations from Table 2 contained a total of 16 unique genes. All of the original DAVID downloads of PE-regulated annotations shown in Tables 1 and 2 and included lists of the genes within an annotation that were also altered by PE. These gene lists were very large and were not included in Tables 1, 2 and 3 to save space. The three annotations “SP_PIR Steroid biosynthesis”, “rno00100 Steroid biosynthesis” and “SP_PIR Sterol biosynthesis” contained 13, 9 and 11 genes, respectively. The 16 genes shown above represent the total unique genes. “Dir”, direction of fold-change. ^aAverage fold-change of 2 or more probesets for a single gene

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