Differential effect of mild and severe pulmonary embolism on the rat lung transcriptome

Zagorski, John; Kline, Jeffrey A.

doi:10.1186/s12931-016-0405-9

Respiratory Research

Table 2 DAVID Functional Annotation Charts, 2-h and 18-h Severe PE verses Vehicle

From: Differential effect of mild and severe pulmonary embolism on the rat lung transcriptome

Category	Term	^a2-h Severe PE verses Vehicle					18-h Severe PE verses Vehicle
Category	Term	Ct	%	Dir	Fold	B&H	Ct	%	Dir	Fold	B&H
SP_PIR	acetylation						183	17.72	UP	1.86	0.000
*SP_PIR*	*activator*	7	*7.29*	UP	*5.58*	*0.025*
*SP_PIR*	*acute phase*						7	*0.68*	UP	*4.89*	*0.047*
*rno00520*	*Amino sugar and nucleotide* *sugar metabolism*						12	*1.16*	UP	*4.37*	*0.002*
rno00970	Aminoacyl-tRNA biosynthesis						12	1.16	UP	4.70	0.002
SP_PIR	Aminoacyl-tRNA synthetase						10	0.97	UP	5.11	0.003
rno00330	Arginine and proline metabolism						11	1.06	UP	3.25	0.046
*rno05412*	*Arrhythmogenic right ventricular* *cardiomyopathy (ARVC)*						15	*0.96*	D	*2.56*	*0.040*
rno05217	Basal cell carcinoma						12	0.77	D	2.97	0.036
IPR011700	Basic leucine zipper	4	*4.17*	UP	*41.80*	*0.004*
IPR004827	Basic-leucine zipper (bZIP) transcription factor	8	8.33	UP	28.54	0.000
SM00338	BRLZ	8	8.33	UP	20.66	0.000
IPR011616	bZIP transcription factor, bZIP-1	4	4.17	UP	25.44	0.015
*SP_PIR*	*Chaperone*						18	*1.74*	UP	*2.78*	*0.006*
SP_PIR	chemotaxis	4	4.17	UP	20.78	0.025	9	0.87	UP	4.43	0.016
*SP_PIR*	*Cholesterol biosynthesis*						9	*0.87*	UP	*7.47*	*0.000*
rno04610	Complement and coagulation cascades						17	1.65	UP	3.80	0.000
SP_PIR	cytokine	9	9.38	UP	15.21	0.000
SP_PIR	DNA binding	6	6.25	UP	7.38	0.028
SP_PIR	dna-binding	15	15.63	UP	3.75	0.002
*UP_SEQ*	*DNA-binding region: Basic motif*	10	*10.42*	UP	*15.44*	*0.000*
*UP_SEQ*	*domain: Leucine-zipper*	9	*9.38*	UP	*19.96*	*0.000*
rno00982	Drug metabolism						20	1.28	D	3.51	0.000
rno04512	ECM-receptor interaction						17	1.09	D	2.65	0.020
*SM00180*	*EGF_Lam*						9	*0.58*	D	*5.44*	*0.020*
*IPR002049*	*EGF-like, laminin*						9	*0.58*	D	*5.98*	*0.026*
*SP_PIR*	*endoplasmic reticulum*						63	*6.10*	UP	*2.04*	*0.000*
PIRSF001719	fos transforming protein	4	4.17	UP	109.2	0.000
IPR000837	Fos transforming protein	4	4.17	UP	83.59	0.001
rno00480	Glutathione metabolism						12	0.77	D	3.03	0.041
rno00340	Histidine metabolism						9	0.58	D	4.73	0.019
SP_PIR	inflammatory response	4	4.17	UP	16.51	0.027	13	1.26	UP	5.08	0.000
*SP_PIR*	*Initiation factor*						12	*1.16*	UP	*4.55*	*0.002*
*SP_PIR*	*Isomerase*						17	*1.65*	UP	*3.14*	*0.003*
*SP_PIR*	*lipid synthesis*						19	*1.84*	UP	*3.82*	*0.000*
rno00980	Metabolism of xenobiotics by cyt. P450						18	1.15	D	3.79	0.000
*UP_SEQ*	*mutagenesis site*						59	*3.77*	D	*1.75*	*0.042*
rno04621	NOD-like receptor signaling pathway	5	5.21	UP	12.52	0.034
*SP_PIR*	*nucleotide-binding*						99	*9.58*	UP	*1.36*	*0.024*
SP_PIR	nucleus	22	22.92	UP	2.01	0.026
*SP_PIR*	*phosphoprotein*						##	*27.49*	UP	*1.24*	*0.000*
*IPR011993*	*Pleckstrin homology-type*						32	*2.05*	D	*2.27*	*0.027*
*SP_PIR*	*protein biosynthesis*						22	*2.13*	UP	*2.04*	*0.049*
*SP_PIR*	*protein transport*						34	*3.29*	UP	*1.87*	*0.016*
*SP_PIR*	*Redox-active center*						10	*0.97*	UP	*4.74*	*0.005*
SP_PIR	ribosome biogenesis						8	0.77	UP	5.31	0.014
*SP_PIR*	*rna-binding*						33	*3.19*	UP	*2.16*	*0.002*
*SM00360*	*RRM*						21	*2.03*	UP	*2.69*	*0.015*
SM00199	SCY	5	5.21	UP	18.26	0.003
IPR001811	Small chemokine, interleukin-8-like	5	5.21	UP	25.22	0.002
*SP_PIR*	*Steroid biosynthesis*						13	*1.26*	UP	*5.08*	*0.000*
*rno00100*	*Steroid biosynthesis*						9	*0.87*	UP	*8.29*	*0.001*
*SP_PIR*	*sterol biosynthesis*						11	*1.06*	UP	*7.31*	*0.000*
*SP_PIR*	*Transcription*	14	*14.58*	UP	*3.23*	*0.009*
*SP_PIR*	*transcription regulation*	14	*14.58*	UP	*3.48*	*0.006*
*SP_PIR*	*translocation*						11	*1.06*	UP	*3.75*	*0.014*
*rno00350*	*Tyrosine metabolism*						10	*0.64*	D	*3.71*	*0.032*

Functional annotations significantly over-represented in the lists of up-regulated and down- regulated genes during 2-h and 18-h High-PE. Annotations unique to High-PE are highlighted in bold italics; annotations common to High-PE and Low-PE (Table 1) are in standard font. All other keys are the same as in Table 1. ^aNo 2-h DOWN annotations meet B&H < 0.05

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ISSN: 1465-993X

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