Fig. 4
From: Weakly acidic pH reduces inflammatory cytokine expression in airway epithelial cells
Inflammatory cytokine expression in AECs “shock treated” for 4 h prior to 24 h LPS stimulation. AECs were incubated in weakly acidic media for 4 h prior to stimulation with LPS in pH7.4 media for 24 h. Expression of inflammatory cytokines IL-6 and IL-8 was measured at the 24 h time point. a Schematic representation of experimental plan. b IL-6 and IL-8 mRNA expression was measured by qPCR. c Secretion of IL-6 and IL-8 protein was measured by ELISA of cell supernatants d Cell viability measured by MTT assay. (n = 3) All test pH conditions were compared to pH7.4 + LPS for statistical analysis. Values presented are mean ± SEM *p < 0.05, **p < 0.01