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Fig. 1 | Respiratory Research

Fig. 1

From: Weakly acidic pH reduces inflammatory cytokine expression in airway epithelial cells

Fig. 1

Epithelial cell response to 24 h incubation in weakly acidic media. AECs were incubated in media adjusted to pH6.5 – pH5.5 with HCl for 24 h (n = 3). Normal, unadjusted BEGM is pH7.4; this was used as a control to show basal IL-6 and IL-8 expression. a Expression of IL-6 and IL-8 mRNA was measured by qPCR. b Corresponding protein secretion was measured in pg/ml by ELISA. c Cell viability was measured by MTT assay. Values presented are mean ± SEM *p < 0.05, **p < 0.01

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