Fig. 6

Alterations in alveolar structure and volume by IAV, RSV or Poly (I:C) in the CS-exposed lungs. a-f Mice were exposed to FA or CS for 4 weeks and instilled with vehicle or (a-c) IAV or (d-f) RSV, and euthanized at day 14 post viral challenge. g-i C57BL/6 mice were exposed to FA or CS for 4 weeks, instilled twice a week with 50 μl of poly(I:C) (50 μg/50 μl) or vehicle on the third and fourth week of CS exposure. Mice were euthanized at the end of 4 weeks of FA or CS exposure. Representative images (a, d, g), morphometric analysis for volume weighted mean alveolar volume (b, e, h), and mean alveolar chord length (c, f, i) of lungs inflated and fixed with formalin under constant pressure. N = 8 mice per group; data reported as mean ± SE; *p < 0.05, **p < 0.01, ***p < 0.001