Table 1 Characterisation of immune cells by flow cytometry
Cell Type | Surface Marker Phenotype | Monoclonal Antibody Conjugate | Catalogue Number | Dilution |
|---|---|---|---|---|
B Cells | CD19+ | CD19-FITC (BD Biosciences) | 557398 | 1/100 |
CD3− | CD3-PECy7 (eBioscience) | 25–0031 | 1/200 | |
Natural Killer (NK) Cells | NKp46+ | NKp46-PE (eBioscience) | 12–3351 | 1/200 |
CD3− | CD3-PECy7 (eBioscience) | 25–0031 | 1/200 | |
CD4+ T Cells | CD4+ | CD4-PerCP (BD Biosciences) | 553052 | 1/200 |
CD3+ | CD3-PECy7 (eBioscience) | 25–0031 | 1/200 | |
CD8+ T Cells | CD8+ | CD8-APC (BD Biosciences) | 553035 | 1/200 |
CD3+ | CD3-PECy7 (eBioscience) | 25–0031 | 1/200 | |
Neutrophils | Ly-6Ghigh | Ly6G-FITC (BD Biosciences) | 551460 | 1/100 |
CD11bhigh | CD11b-PerCP (eBioscience) | 45–0112 | 1/400 | |
CD11clow | CD11c-APC (BD Biosciences) | 550261 | 1/200 | |
F4/80low | F4/80-PE (eBioscience) | 12–4801 | 1/50 | |
Alveolar Macrophages | CD11blow-int. | CD11b-PerCP (eBioscience) | 45–0112 | 1/400 |
CD11chigh | CD11c-APC (BD Biosciences) | 550261 | 1/200 | |
F4/80high | F4/80-PE (eBioscience) | 12–4801 | 1/50 | |
Inflammatory monocytes/ macrophages | CD11bhigh | CD11b-PerCP (eBioscience) | 45–0112 | 1/400 |
CD11clow | CD11c-APC (BD Biosciences) | 550261 | 1/200 | |
F4/80high | F4/80-PE (eBioscience) | 12–4801 | 1/50 | |
Eosinophils | CD11bhigh | CD11b-PerCP (eBioscience) | 45–0112 | 1/400 |
CD11clow | CD11c-APC (BD Biosciences) | 550261 | 1/200 | |
SiglecFhigh | SiglecF-PE (BD Biosciences) | 552126 | 1/200 |