Fig. 5

Changes in plasma α1-AT abundance and bioactivity after instillation of whole gastric fluid. a Representative Western blot of plasma samples illustrating the time course of changes in plasma α1-AT abundance. The ~55 kDa α1-AT immunoreactive band seen in the control sample shows a significant enlargement at 24 h. b Densitometric analysis of Western blots, normalized by their corresponding Ponceaus and expressed as times over control values (n = 5 for each time point). α1-AT immunoreactivity increased 1.5 times the control values at 24 h and was back to control levels at 4d. c Plasma α1-AT bioactivity increased 1.3 times with respect to control values at 24 h with return to control values at 4d. Bars are means ± 1SD; **: p < 0.01 with respect to control values. C: controls; h: hours; d: days