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Fig. 5 | Respiratory Research

Fig. 5

From: Investigating the role of MRGPRC11 and capsaicin-sensitive afferent nerves in the anti-influenza effects exerted by SLIGRL-amide in murine airways

Fig. 5

Semiquantitative measurement of anti-IAV activities of SLIGRL-amide and BAM8-22 in mouse isolated tracheal segments by immunohistochemical staining of IAV nucleoprotein. a Immunohistochemical staining for IAV nucleoprotein in mouse tracheal sections. Mouse tracheal segments were mounted onto an airway explant perfusion system and incubated for 15 mins with IAV in the presence or absence of SLIGRL-amide or BAM8-22. Some tracheal segments were exposed to dilute allantoic fluid instead of IAV (No IAV). After 48 h of perfusion with media, segments were fixed, paraffin-embedded, sectioned and immunohistochemically stained for IAV. Shown are representative images from at least 3 experiments. Bar = 50 μm. b Levels of staining for immunoreactive-IAV in the epithelium of perfused trachea exposed to IAV in the absence (n = 29 trachea) or presence of SLIGRL-amide (n = 8 trachea) or BAM8-22 (n = 4 trachea). Some tracheal segments were exposed to dilute allantoic fluid instead of IAV (No IAV; n = 3 trachea). Shown are mean levels of immunoreactive IAV staining (and standard error of the mean), expressed as % of strong positive pixels, as outlined in the Methods section. *, indicates P <0.05, compared to IAV + saline control (one-way ANOVA)

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