Fig. 4
From: Ajulemic acid exerts potent anti-fibrotic effect during the fibrogenic phase of bleomycin lung
AjA treatment is followed by enhanced PPAR-γ expression, reduced pSMAD2/3 nuclear localization and reduced expression of CTGF. a Representative immunohistochemical staining of PPAR-γ in lung section from animals of the different experimental groups at 14 days after BLM delivery. Scale bars = 60 μm. b Immunohistochemical staining of pSMAD2/3 in lung section from control mouse, BLM-treated mouse and mice receiving in the same day of BLM delivery AjA at a dose of 1 mg/Kg and 5 mg/Kg, at 14 days after BLM delivery. Scale bars = 60 μm. c Representative immunohistochemical staining of CTGF in lung section from the different experimental groups. Scale bars = 60 μm. d–f Immunohistochemical scores relative to cytoplasmic and nuclear localization of PPAR-γ, pSMAD2/3 and CTGF positive cells from the various experimental groups analysed in the preventive study. Data are reported as mean ± SD. *p < 0.05 vs control group. † p < 0.05 vs BLM group