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Fig. 6 | Respiratory Research

Fig. 6

From: Airway epithelial cell exposure to distinct e-cigarette liquid flavorings reveals toxicity thresholds and activation of CFTR by the chocolate flavoring 2,5-dimethypyrazine

Fig. 6

Increased intracellular cAMP dampens 2,5-dimethylpyrazine-evoked apical ion efflux. Primary MTE cells were voltage clamped in Ussing chambers and monitored for changes in Isc and TER following addition of 0.2 % v/v 2,5-dimethylpyrazine. a Representative traces of Isc and b TER (left) from MTE cells pre-treated with 8-bromo-cAMP (dotted arrow) followed by amiloride (dashed arrow) and 2,5-dimethylpyrazine (solid arrow). Quantification of Isc and TER (A-B right) of amiloride controls (n = 3) versus cells treated with 8-bromo-cAMP and amiloride (n = 3) are plotted. The addition of the cAMP analog 8-bromo-cAMP significantly reduced apical ion efflux evoked by 2,5-dimethypyrazine suggesting it activates a receptor that increases cAMP production. *indicates significant difference P < 0.05 in a one-way ANOVA with a Dunnett’s Multiple Comparison Test

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