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Fig. 4 | Respiratory Research

Fig. 4

From: Airway epithelial cell exposure to distinct e-cigarette liquid flavorings reveals toxicity thresholds and activation of CFTR by the chocolate flavoring 2,5-dimethypyrazine

Fig. 4

2,5-Dimethylpyrazine activates apical ion efflux via CFTR. Primary MTE cells were voltage clamped in Ussing chambers and monitored for changes in Isc and TER following addition of 0.2 % v/v 2,5-dimethylpyrazine. a Representative traces of Isc and b TER (left) from MTE cells treated with 2,5-dimethylpyrazine alone (solid line) or 2,5-dimethylpyrazine plus CFTR-172 inh [amiloride (dashed arrow), CFTR-172 inh (dotted arrow), 2,5-dimethylpyrazine (solid arrow)]. Quantification of Isc and TER (A-B right) of amiloride controls (n = 3) versus cells treated with amiloride and CFTR-172 inh (n = 4) are graphed. The addition of CFTR-172 inh significantly reduced the 2,5-dimethypyrazine-evoked apical ion efflux suggesting Cl secretion via CFTR. *indicates significant difference P < 0.05 in a one-way ANOVA with a Dunnett’s Multiple Comparison Test

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