Fig. 5

The WNT10A expression regulated collagen production. a Cells were treated with TGF-β1 (5 ng/ml), and at the indicated time points, the mRNA level of COL1A1 was assessed by real-time qPCR. ^* p < 0.05. b Collagen production was measured 24 h after TGF-β1 (5 ng/ml) stimulation. ^* p < 0.05. c The efficacy of WNT10A silencing was evaluated by immunoblotting for β-catenin and WNT10A proteins in IMR-90 and LL97A cells. ^* p < 0.05. d LL97A and IMR-90 cells transfected with indicated siRNAs for 72 h were treated with TGF-β1 (5 ng/ml). After 24 h, the mRNA level of COL1A1 was assessed by real-time qPCR. ^* p < 0.05. All results are expressed as the means ± SEM