Fig. 4

BLM induces WNT10A and β-catenin expressions. a The mRNA level of WNT10A and β-catenin in BLM-treated and control mice was assessed by real-time qPCR (n = 10 each). *p < 0.05. b Lung tissue specimens of BLM-treated and control mice were stained with anti-WNT10A and β-catenin antibodies. The arrow indicates the WNT10A and β-catenin expressions in fibroblasts. c COS1 cells stably transfected with WNT10A-luciferase reporter plasmid which can replicate in the cells were treated with the indicated concentration of BLM. After 24 h, a luciferase assay was performed and the results are expressed as the means ± SEM. ^* p < 0.05. d The transfectants described in (c) were treated with 50 uM BLM for the indicated times, and then were changed to a new medium. After 48 h, a luciferase assay was performed as a recovery assay, and the results are expressed as the means ± SEM. ^* p < 0.05