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Table 1 Mixed leukocyte reaction (MLR)

From: Human lung fibroblasts may modulate dendritic cell phenotype and function: results from a pilot in vitro study

Conditions

MUTZ-3 DC medium

Contact Co-culture

medium

Control Fibro

IPF Fibro

Lymphocyte Proliferation (mean cpm ± SD, n = 3)

56,870 ± 6110

40,236 ± 6047

34,322 ± 2843

p value (versus MUTZ-3 DC)

 

0.02

0.01

Conditions

MUTZ-3 DC medium

Transwell Co-culture

medium

Control Fibro

IPF Fibro

Lymphocyte Proliferation (mean cpm ± SD, n = 3)

36,967 ± 4942

29,328 ± 3215

26,890 ± 6564

p value (versus MUTZ-3 DC)

0.04

0.05

  1. MLR was performed by culturing with 100 000 lymphocytes (depleted of adherent mononuclear cells by a 2 h-lasting culture) and 20 000 MUTZ-3 DCs previously cultured alone (medium) or co-cultured with control or IPF fibroblasts for 48 h (as in Fig. 1) in 200 μl RPMI complete medium (n = 6 for each condition) for 6 days During the last 18 h, 1 μCi of 3H-thymidine (Perkin-Elmer Wallac) was added per well. Incorporated radioactivity was determined using a MicroBeta plate reader (Wallac WS-Trilux 1450
  2. Results are presented as mean (±SD) lymphocyte proliferation induced by MUTZ-3 DCs previously alone (medium) or co-cultured with control or IPF fibroblasts either in direct contact or in transwell conditions during 48 h before MLR
  3. 3H-thymidine incorporation of 105 lymphocytes alone was 605 ± 436 cpm and 3H-thymidine incorporation of 20 000 MUTZ3 DC alone was 138 ± 59 cpm, (mean ± SD, n = 6)