Fig. 2

Adhesion Receptor Expression on Pulmonary Leukocytes at 3 Months. Leukocytes from perfused lungs were analyzed by FACS to quantify leukocyte subsets and their receptor expression. a, b At 3 months, there was a trend but no significant increase in total lung leukocytes in HFD mice. There was no significant change in leukocyte subsets at 3 months shown as % CD45⁺ cells. c, d By 6 months, there was a significant increase in total leukocytes isolated from the digested lung from HFD vs. LFD mice, and these changes were comprised of increases in CD3⁺ lymphocytes and Ly6C^low monocytes. e Gating strategy to identify lung PMNs, alveolar macrophages and monocyte populations. f PMN adhesion and chemokine receptor expression at 3 and 6 months demonstrate early and persistent increases in LFA-1 and ICAM-1 expression in HFD mice. CXCR2 expression was not significantly reduced until 6 months on a HFD. g Histograms for ICAM-1 expression demonstrate 2 PMN populations in the lung compartment, with an increase in the proportion of ICAM-1⁺ PMNs in HFD mice at 3 and 6 months. h Ly6C^high monocyte adhesion receptor expression at 3 and 6 months demonstrate early and sustained increase in LFA-1 expression and later increases in CD62L and ICAM-1 expression in HFD vs. LFD mice. Alveolar macrophage (AM), Eosinophils (Eos). *p value < 0.05; mean ± SEM (n = 5 mice/group, experimental replicates 3)