Fig. 1

Circulating leukocytes after 3 months on a high fat (HFD) vs. low fat diet (LFD). Blood leukocytes were analyzed by FACS to quantify leukocytes subsets and their receptor expression. a Polymorphonuclear leukocytes (PMNs) were identified by Ly6G^posCXCR2^pos staining (blue circle). b After excluding PMNs, CD11b⁺SSC^low leukocytes were selected to identify Ly6C^low (grey circle) and Ly6C^high (red circle) monocytes (monos). c HFD mice had greater numbers of total white blood cells (WBCs) in the blood, comprised of lymphocytes (lymphs) and monocytes. d HFD PMNs expressed greater levels of LFA-1, but not CD62L or CXCR2. e HFD Ly6C^high monocytes expressed higher levels of LFA-1, CD62L and ICAM-1. f HFD Ly6C^low monocytes did not have a significant difference in LFA-1 or ICAM-1 expression. Mean fluorescent intensity (MFI). *p value < 0.05; mean ± SEM (n = 5 mice/group, experimental replicates 2)