Fig. 4

BLM-induced pulmonary fibrosis in periostin-CNP Tg mice. BLM (1 mg/kg) was administered intratracheally to periostin-CNP Tg mice and WT littermates on day 0, and the lung tissues were removed on day 14. Representative micrographs of lung tissue stained with hematoxylin-eosin (HE; upper panels) or Masson trichrome (MT; lower panels); WT littermates without BLM administration (a, e), periostin-CNP Tg mice without BLM administration (b, f), WT littermates with BLM administration (c, g), and periostin-CNP Tg mice with BLM administration (d, h) are shown. Scale bar: 500 μm. i Fibrotic area was analyzed by using image analyzing software and is expressed as a percentage of the whole lung field. Values represent means ± SEM (WT without BLM, n = 7; Tg without BLM, n = 7; WT with BLM, n = 8; Tg with BLM, n = 9). *P < 0.05. NA, not assessed because of no fibrotic area in normal lungs. j Lung weight. Values represent means ± SEM (WT without BLM, n = 7; Tg without BLM, n = 5; WT with BLM, n = 7; Tg with BLM, n = 5). *P < 0.05