Fig. 3
From: C-type natriuretic peptide ameliorates pulmonary fibrosis by acting on lung fibroblasts in mice
Generation of the periostin-C-type natriuretic peptide transgenic mouse (periostin-CNP Tg mouse). a Schematic representation of the periostin-CNP transgene. The transgene consisted of the periostin promoter (containing the 5′-untranslated region, shown as a gray box) linked to the human CNP gene (exons indicated by closed boxes) and an SV40 polyadenylation sequence. The probe (855 bp) used for Southern blot analysis is indicated by a double-headed arrow. b Southern blot analysis of EcoRV-digested mouse genomic DNA from a periostin-CNP Tg mouse and its wild-type (WT) littermate hybridized with the probe for hCNP transgene indicated in Fig. 3a. c The expression of the transgene was examined in aorta, heart, lung, kidney, liver, and brain by reverse transcribed–PCR. Tg, periostin-CNP transgenic mouse; WT, wild-type littermate. d The mRNA expression levels of the transgene and GC-B receptor were examined in primary cultured lung fibroblasts from a periostin-CNP Tg mouse and its WT littermate by PCR. A representative image of 3 independent experiments is shown. e The concentration of CNP in plasma, aorta, heart, and lung (WT, n = 3; Tg, n = 3). *P < 0.05. ND, not detected. f Body weight and weights of lung, heart and kidney. Values represent means ± SEM (WT, n = 7; Tg, n = 5)