Fig. 7

EMMPRIN Overexpression Increases Wnt/β-catenin signaling: (a) Representative Western blot for phosphorylated β-catenin of cells lysates from NHLF overexpressing either EMMPRIN/GFP or GFP treated with and without TGF-β1 (10 ng/ml). β-Tubulin was used as the loading control. Image is representative of 3 independent experiments, each run in duplicate. b Transient transfection of TOPFLASH, FOPFLASH, and control renilla luciferase reporter constructs into NHLF overexpressing either EMMPRIN/GFP or GFP then treated with TGF-β1 10 ng/ml. Each bar represents means ± SEM of 3 independent experiments, each run in duplicate, ** p < 0.001comparing GFP to EMMPRIN/GFP transfected cells