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Table 2 Mean EC50, IC50 and Emax for dexamethasone alone and in combination with PDE4 inhibitors in blood CD8 cells and PBMCs

From: Additive anti-inflammatory effects of corticosteroids and phosphodiesterase-4 inhibitors in COPD CD8 cells

  Dex Dex/256066 Dex/Rof Dex/Forsk
  Mean EC50 IL-2
CD8 COPD 7.8 4.8 6.7 1.6
Smoker 16.6 5.4 6.1 14.1
HNS 11.2 5.4 7.7 2.7
PBMC COPD 8.91 11.4 4.2 2.2
Smoker 22.9 8.3 8.7 18.2
HNS 12.0 4.9 3.8 2.0
  Mean EC50 IFNγ
CD8 COPD 10.0 4.0 5.0 1.6
Smoker 20.0 6.3 7.9 10.0
HNS 15.8 12.6 3.2 5.0
PBMC COPD 31.6 4.0 6.3 10.0
Smoker 20.0 10.0 7.9 15.8
HNS 10.0 4.0 2.5 2.5
  Mean IC50 IL-2
CD8 COPD 7.9 5.5 7.1 1.8
Smoker 16.6 6.2 8.5 22.9
HNS 7.9 5.9 8.3 3.2
PBMC COPD 8.5 30.2 4.8 1.6
Smoker 22.9 6.6 9.3 33.9
HNS 10.7 5.5 6.5 3.1
  Mean IC50 IFNγ
CD8 COPD 12.0 3.6 4.6 1.9
Smoker 18.2 8.1 7.4 11.0
HNS 21.4 8.1 3.6 0.8
PBMC COPD 33.1 5.0 8.5 9.8
Smoker 23.4 8.7 8.9 24.0
HNS 15.1 6.0 3.6 1.0
  Emax IL2
CD8 COPD 67.9 78.1 78.2 86.6
Smoker 68.5 86.5 81.9 80.9
HNS 66.3 82.0 80.7 79.7
PBMC COPD 64.2 87.4 83.0 81.6
Smoker 61.1 84.8 79.2 84.1
HNS 61.9 84.3 75.9 77.5
  Emax IFNγ
CD8 COPD 59.8 74.9 75.1 78.8
Smoker 59.2 77.6 72.9 76.6
HNS 56.3 70.5 65.4 75.4
PBMC COPD 69.7 86.4 82.2 85.7
Smoker 66.0 84.0 83.6 75.4
HNS 61.0 83.2 82.9 73.7
  1. Data presented mean nanomolar concentration for EC50 and IC50. Data presented as mean percent inhibition for Emax. Dex: dexamethasone; 256066: GSK256066;CD8: circulating CD8 cells; PBMC: peripheral blood mononuclear cells; Rof: roflumilast; Forsk: forskolin; COPD: chronic obstructive pulmonary disease; HNS: healthy non-smoker; IL-2: interleukin 2; IFNγ: interferon gamma; EC50:concentration producing 50 % of maximal effect; IC50: concentration producing reduction of 50 % relative to untreated stimulated levels; Emax: Maximal inhibitory effect at dexamethasone 10−6 M