Skip to main content

Table 2 Mean EC50, IC50 and Emax for dexamethasone alone and in combination with PDE4 inhibitors in blood CD8 cells and PBMCs

From: Additive anti-inflammatory effects of corticosteroids and phosphodiesterase-4 inhibitors in COPD CD8 cells

 

Dex

Dex/256066

Dex/Rof

Dex/Forsk

 

Mean EC50 IL-2

CD8

COPD

7.8

4.8

6.7

1.6

Smoker

16.6

5.4

6.1

14.1

HNS

11.2

5.4

7.7

2.7

PBMC

COPD

8.91

11.4

4.2

2.2

Smoker

22.9

8.3

8.7

18.2

HNS

12.0

4.9

3.8

2.0

 

Mean EC50 IFNγ

CD8

COPD

10.0

4.0

5.0

1.6

Smoker

20.0

6.3

7.9

10.0

HNS

15.8

12.6

3.2

5.0

PBMC

COPD

31.6

4.0

6.3

10.0

Smoker

20.0

10.0

7.9

15.8

HNS

10.0

4.0

2.5

2.5

 

Mean IC50 IL-2

CD8

COPD

7.9

5.5

7.1

1.8

Smoker

16.6

6.2

8.5

22.9

HNS

7.9

5.9

8.3

3.2

PBMC

COPD

8.5

30.2

4.8

1.6

Smoker

22.9

6.6

9.3

33.9

HNS

10.7

5.5

6.5

3.1

 

Mean IC50 IFNγ

CD8

COPD

12.0

3.6

4.6

1.9

Smoker

18.2

8.1

7.4

11.0

HNS

21.4

8.1

3.6

0.8

PBMC

COPD

33.1

5.0

8.5

9.8

Smoker

23.4

8.7

8.9

24.0

HNS

15.1

6.0

3.6

1.0

 

Emax IL2

CD8

COPD

67.9

78.1

78.2

86.6

Smoker

68.5

86.5

81.9

80.9

HNS

66.3

82.0

80.7

79.7

PBMC

COPD

64.2

87.4

83.0

81.6

Smoker

61.1

84.8

79.2

84.1

HNS

61.9

84.3

75.9

77.5

 

Emax IFNγ

CD8

COPD

59.8

74.9

75.1

78.8

Smoker

59.2

77.6

72.9

76.6

HNS

56.3

70.5

65.4

75.4

PBMC

COPD

69.7

86.4

82.2

85.7

Smoker

66.0

84.0

83.6

75.4

HNS

61.0

83.2

82.9

73.7

  1. Data presented mean nanomolar concentration for EC50 and IC50. Data presented as mean percent inhibition for Emax. Dex: dexamethasone; 256066: GSK256066;CD8: circulating CD8 cells; PBMC: peripheral blood mononuclear cells; Rof: roflumilast; Forsk: forskolin; COPD: chronic obstructive pulmonary disease; HNS: healthy non-smoker; IL-2: interleukin 2; IFNγ: interferon gamma; EC50:concentration producing 50 % of maximal effect; IC50: concentration producing reduction of 50 % relative to untreated stimulated levels; Emax: Maximal inhibitory effect at dexamethasone 10−6 M