Skip to main content
Fig. 5 | Respiratory Research

Fig. 5

From: Additive anti-inflammatory effects of corticosteroids and phosphodiesterase-4 inhibitors in COPD CD8 cells

Fig. 5

The effect of dexamethasone and PDE4 inhibitors of nuclear translocation of phoshporylated-CREB. Peripheral blood CD8 cells were isolated from 4 COPD patients. Cells were treated with GSK256066 (10−9 M) or forskolin (10−6 M) alone or dexamethasone (10−6 M) alone and in combination with GSK256066 or forskolin. Cells were immunostained with rabbit anti-phospho-cAMP response element binding (pCREB, red). Nuclei were counterstained with 4′,6-diamidino-2-phenylindole (blue). The nuclear mean fluorescence intensity (MFI) of pCREB was measured. Representative images of each experimental condition (a-g). Data is presented as mean ± SE pCREB nuclear MFI (H). Statistically significant increase in nuclear pCREB is indicated when * p < 0.05

Back to article page