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Fig. 4 | Respiratory Research

Fig. 4

From: Additive anti-inflammatory effects of corticosteroids and phosphodiesterase-4 inhibitors in COPD CD8 cells

Fig. 4

Effect of Dexamethasone and PDE4 inhibitors on nuclear translocation of Glucocorticoid Receptor. Peripheral blood CD8 cells from COPD patients (n = 5) were untreated (basal), treated with GSK256066 (10−9 M), roflumilast (10−7 M), or forskolin (10−6 M) alone or treated with dexamethasone (10−6 M, Dex) alone and in combination with GSK256066, roflumilast or forskolin. Cells were harvested after 60 min. Cells were immunostained with rabbit anti-glucocorticoid receptor (GR) antibody (red). Nuclei were counterstained with 4′,6-diamidino-2-phenylindole (blue). The nuclear mean fluorescence intensity (MFI) of GR was measured. Representative images of each experimental treatment are shown (a-g). h Data is presented as mean ± SE GR nuclear MFI normalised to basal levels. Statistically significant increase in nuclear GR above basal is indicated when *p < 0.05

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