Fig. 3

The effect of GSK256066 with dexamethasone on cytokine release from pulmonary CD8 cells. Pulmonary CD8 cells from COPD (n = 6) and smokers with normal lung function (n = 4) were pre-treated with various concentrations of dexamethasone (Dex) alone (О) or in combination with GSK256066 (■). The effect of GSK256066 alone is represented as log[Dex]M = 0. Cells were then stimulated with anti-CD2/3/28 beads for 24 h. Supernatants were harvested and interleukin 2 (IL-2) and interferon gamma (IFNγ) were measured by ELISA. Data presented as mean ± 95 % confidence intervals. a COPD, IL-2, b Smokers, IL-2, c COPD, IFNγ, d Smoker. IFNγ. Statistical significance of difference between dexamethasone alone compared to dexamethasone with GSK256066 indicated by * p < 0.05