Fig. 5

Change in IgG₄ levels and affinity in improved and non-improved patients. a Levels of circulating IgG₄ in improved and non-improved actively treated patients, as well as placebo patients, before treatment, four weeks after treatment and following the consecutive pollen season. Analysis was performed using a repeated measures ANOVA. b IgG₄ affinity in improved and non-improved patients, measured and presented as percent allergen-bound IgG₄ with increasing concentrations of ammonium thiocyanate (NH₄SCN). n = 3–7 *p < 0.05 using two-way ANOVA followed by Fisher’s LSD