Fig. 7
Effects of KLF5 silencing by siRNA on the ONOO−-augmented release of matrix metalloproteinases (MMPs). Effects of KLF5 silencing on the ONOO−-augmented release of MMP-9 and MMP-2 were investigated by gelatin zymography (a). After silencing, the infected cells were incubated with 10−7 M ONOO− (filled bars) or vehicles (open bars) for 24 h. Latent form of MMP-9 (a and b), active form of MMP-9 (a and c), latent form of MMP-2 (a and d), and active form of MMP-2 were evaluated. Each band intensity was analyzed by Quantity One. All values are expressed as mean ± SD for 4 separate experiments. **p < 0.01 versus vehicle-pretreated vehicle-exposed group. † p < 0.05, †† p < 0.01 versus vehicle-pretreated ONOO−-exposed group