Fig. 6

Effects of KLF5 silencing by siRNA on the H₂O₂ or ONOO⁻-augmented the production of α-smooth muscle actin (α-SMA) and release of collagens. Effects of siRNA for KLF5 on the gene expression and the protein production were confirmed by quantitative reverse transcription-polymerase chain reaction (qRT-PCR) for 24 h after transfection (a) and immunoblotting for 72 h (b). Effects of KLF5 silencing on the H₂O₂ (c) or the ONOO⁻ (d)-augmented production of α-SMA were analyzed by immunoblotting. Each band intensity was analyzed by Quantity One. Effect of KLF5 silencing on the ONOO⁻-augmented release of collagens was analyzed (e). All values are expressed as mean ± SD for 4 separate experiments. **p < 0.01 versus vehicle-pretreated vehicle-exposed group. ^† p < 0.05, ^†† p < 0.01 versus vehicle-pretreated H₂O₂ or ONOO⁻-exposed group