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Fig. 5 | Respiratory Research

Fig. 5

From: Possible role of Krüppel-like factor 5 in the remodeling of small airways and pulmonary vessels in chronic obstructive pulmonary disease

Fig. 5

Effect of hydrogen peroxide (H2O2) or peroxynitrite (ONOO) on KLF5 production and translocation into the nucleus in human fetal lung fibroblasts (HFL-1). Production of KLF5 was analyzed by immunoblotting. HFL-1 cells were treated with various concentrations of H2O2 for 24 h (a) and with 10−6 M H2O2 for various durations (b) as well as ONOO for 24 h (c) and 10−7 M ONOO for various durations (d). Each band intensity of KLF5 was standardized by that of β-actin. Translocation of KLF5 into the nucleus was also analyzed by immunoblotting. The cells were treated with 10−6 M H2O2 (e) or 10−7 M ONOO (f) and the nuclear fraction was harvested at various time points. Each band intensity of KLF5 was standardized by that of lamin A/C. All values are expressed as mean ± SD for 4–7 separate experiments. *p < 0.05, **p < 0.01 versus control group

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