Fig. 3From: Possible role of Krüppel-like factor 5 in the remodeling of small airways and pulmonary vessels in chronic obstructive pulmonary diseaseImmunoreactivity of KLF5 in the bronchial fibroblasts. To determine the cell types that expressed KLF5, double immunostaining for KLF5 and prolyl 4-hydroxylase β (P4HB) was carried out. P4HB is a marker of fibroblasts. Representative photographs of the immunoreactivity of KLF5 (green) and P4HB (red) are shown (original magnification × 200 for all panels; scale bars = 100 μm). A negative control sample was provided to replace the non-specific polyclonal rabbit IgG antibody instead of the primary antibody for the lung tissue specimens of the patients with COPD. DAPI: 4′,6-diamidino-2-phenylindole; HE: hematoxylin-eosin stainingBack to article page