Fig. 7
From: IL-25 and IL-33 induce Type 2 inflammation in basophils from subjects with allergic asthma
Blocking βc neutralizes the effects of airway samples on basophils. Purified peripheral blood basophils from 16 mild allergic asthmatic subjects were suspended in RPMI-C and cultured for 18 h with PBS as negative control (open bars) or 7 h post-allergen sputum supernatant and neutralizing antibodies to (a) TSLPR, (b) ST2 or IL-17RB, and (a, b) βc (10 μg/mL) (light grey bars) on markers of basophil function by expression of CD203c, IL-4, and IL-13. Data expressed as mean ± SEM. IL-3 (10 ng/mL) served as positive control (dark greys bars) *Significantly different from PBS negative control, †significantly different from isotype control (open bars); P < 0.05 (N = 8)